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SRX25513467: whole-genome sequencing of chikungunya virus vaccine strain to examine RdRP fidelity in nsP4 variants
1 ILLUMINA (NextSeq 2000) run: 480,000 spots, 44.5M bases, 14.9Mb downloads

Design: viral rna extraction followed by random-primed cdna synthesis and on-bead tagmentation with illumina S beads and library amplification with EPM. paired end reads quality trimmed and merged
Submitted by: Greninger (Alex)
Study: Deep sequencing of chikungunya virus vaccine strain 181/25 cultures with nsP4 variants
PRJNA1141983 • SRP523089 • All experiments • All runs
show Abstracthide Abstract
Data reported in Mutations in Chikungunya Virus nsP4 Decrease Sensitivityto the Broad-Spectrum Antiviral 4'-Fluorouridine. Chikungunya virus vaccine strain 181/25 with nsp4 C483 and nsp4 Y483 were cultured, RNA extracted, and deep sequenced to measure low-frequency mutations to compare fidelity of these RdRP variants.
Sample:
SAMN42914207 • SRS22167673 • All experiments • All runs
Organism: Chikungunya virus
Library:
Name: 231018_Einstein_PY_007_U2OS_C483Y_1
Instrument: NextSeq 2000
Strategy: WGS
Source: VIRAL RNA
Selection: cDNA_randomPriming
Layout: SINGLE
Runs: 1 run, 480,000 spots, 44.5M bases, 14.9Mb
Run# of Spots# of BasesSizePublished
SRR30037987480,00044.5M14.9Mb2024-07-30

ID:
34495099

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